Review



pser pkc substrate  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pser pkc substrate
    Western blot analysis was performed on whole cerebellar homogenate from all genotypes (WT, yellow; HET, red; HOM, purple) and sexes (N=6-11 mice per group) to assess <t>pSer</t> <t>PKC</t> substrate phosphorylation, GSK3β (Ser 9 ) phosphorylation, and PKCδ and PKCη expression. Western blot analysis for a) pSer PKC substrate phosphorylation sites, b) phosphorylated over total GSK3β, c) PKCδ, and d) PKCη identified no significant differences between genotype or sex. Western blot data is normalized to WT. Bar graphs represented quantification of mean±S.E.M. Significance was determined by One-way ANOVA with Tukey’s post hoc.
    Pser Pkc Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pser pkc substrate/product/Cell Signaling Technology Inc
    Average 96 stars, based on 564 article reviews
    pser pkc substrate - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14"

    Article Title: Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14

    Journal: bioRxiv

    doi: 10.1101/2025.02.10.637267

    Western blot analysis was performed on whole cerebellar homogenate from all genotypes (WT, yellow; HET, red; HOM, purple) and sexes (N=6-11 mice per group) to assess pSer PKC substrate phosphorylation, GSK3β (Ser 9 ) phosphorylation, and PKCδ and PKCη expression. Western blot analysis for a) pSer PKC substrate phosphorylation sites, b) phosphorylated over total GSK3β, c) PKCδ, and d) PKCη identified no significant differences between genotype or sex. Western blot data is normalized to WT. Bar graphs represented quantification of mean±S.E.M. Significance was determined by One-way ANOVA with Tukey’s post hoc.
    Figure Legend Snippet: Western blot analysis was performed on whole cerebellar homogenate from all genotypes (WT, yellow; HET, red; HOM, purple) and sexes (N=6-11 mice per group) to assess pSer PKC substrate phosphorylation, GSK3β (Ser 9 ) phosphorylation, and PKCδ and PKCη expression. Western blot analysis for a) pSer PKC substrate phosphorylation sites, b) phosphorylated over total GSK3β, c) PKCδ, and d) PKCη identified no significant differences between genotype or sex. Western blot data is normalized to WT. Bar graphs represented quantification of mean±S.E.M. Significance was determined by One-way ANOVA with Tukey’s post hoc.

    Techniques Used: Western Blot, Expressing



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    Image Search Results


    Western blot analysis was performed on whole cerebellar homogenate from all genotypes (WT, yellow; HET, red; HOM, purple) and sexes (N=6-11 mice per group) to assess pSer PKC substrate phosphorylation, GSK3β (Ser 9 ) phosphorylation, and PKCδ and PKCη expression. Western blot analysis for a) pSer PKC substrate phosphorylation sites, b) phosphorylated over total GSK3β, c) PKCδ, and d) PKCη identified no significant differences between genotype or sex. Western blot data is normalized to WT. Bar graphs represented quantification of mean±S.E.M. Significance was determined by One-way ANOVA with Tukey’s post hoc.

    Journal: bioRxiv

    Article Title: Sex specific disruptions in Protein Kinase Cγ signaling in a mouse model of Spinocerebellar Ataxia Type 14

    doi: 10.1101/2025.02.10.637267

    Figure Lengend Snippet: Western blot analysis was performed on whole cerebellar homogenate from all genotypes (WT, yellow; HET, red; HOM, purple) and sexes (N=6-11 mice per group) to assess pSer PKC substrate phosphorylation, GSK3β (Ser 9 ) phosphorylation, and PKCδ and PKCη expression. Western blot analysis for a) pSer PKC substrate phosphorylation sites, b) phosphorylated over total GSK3β, c) PKCδ, and d) PKCη identified no significant differences between genotype or sex. Western blot data is normalized to WT. Bar graphs represented quantification of mean±S.E.M. Significance was determined by One-way ANOVA with Tukey’s post hoc.

    Article Snippet: Antibodies used are listed with the company from which they were purchased and catalog number: PKCα (BDT, cat. no. 610108), pSer PKC substrate (Cell Signaling, cat. no. 2261, lot 26), PKCγ (Santa Cruz, cat. no. C-19; or GTX, cat. no. 107639), PKCα (BD Transduction, cat. no. 610108; or Santa Cruz Bio, cat. no. sc-8393), α-Tubulin (Sigma Aldrich, cat. no. T6074), Calbindin D28k (Swant, cat. no. 300), PKCδ (BD, cat. no. 610397), phospho-GSK3 α/β(Ser21/Ser9) (Cell Signaling, cat. no. 9331), total GSK3 α/β (Cell Signaling, cat. no. 9832), phospho-MARCKS (Ser159/Ser163) (Cell Signaling, cat. no. 11992), total MARCKS (Proteintech cat. no. 20661), Vinculin (Cell Signaling, cat. no. 4650), PKCη (Abcam, cat. no. 179542), GAPDH (Cell Signaling, cat. no. 2118), Goat anti-mouse, Azure700 conjugate (Azure Biosystems, cat. no. AC2129), and Goat anti-rabbit, Azure800 conjugate (Azure Biosystems, cat. no. AC2134).

    Techniques: Western Blot, Expressing